C (3-6 months)
true
<A HREF="https://mus.brc.riken.jp/en/mouse_of_month/feb_2019_mm" target="_blank">Mouse of the Month Feb 2019</A>
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 63, 311-319 (2014).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR Dr. Yoshihiro Miwa (ymiwa@md.tsukuba.ac.jp) of University of Tsukuba using the Approval Form.
Transgenic mice expressing iRFP under the control of the CAG promoter. This line (B6.Cg-c/c Tg(CAG-iRFP)#867Utr) exhibits stronger iRFP expression in tissues other than heart, compared to RBRC10067 (B6.Cg-c/c Tg(CAG-iRFP)#846Utr).
2012年にBDF1で作製。開発者／機関：三輪佳宏／筑波大学開発年：2012年入手経路等：C57BL/6Jは日本SLCより入手B6(Cg)Tyr<c2-J>/JはJackson Laboratoryより入手iRFP遺伝子は新学術領域研究「蛍光生体イメージ」より供与。BDF1で作製後、寄託までにB6(Cg)Tyr<c2-J>/Jに17回戻し交配を行い、transgeneをヘテロで維持し続けた。全身性に全臓器でiRFPを発現する。
Developed by Yoshihiro Miwa, University of Tsukuba in 2012. A DNA construct was injected into fertilized eggs derived from B6D2F1. Mice were backcrossed to B6(Cg)Tyr<c2-J> for 17 generations. C57BL/6J congenic line.
C（3〜6か月）
Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Rhodopseudomonas palustris iRFP DNA, SV40 Poly(A) site
Fluorescent Proteins/lacZ System
B6.Cg-c/c Tg(CAG-iRFP)#867Utr
CAG promoterより全身性に蛍光タンパク質iRFPを発現する。特に心臓以外の臓器組織でB6.Cg-Tg(CAG-iRFP) #846より強く発現している。
条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Exp. Anim., 63, 311-319 (2014).<br>学術機関の学術研究に限る。利用者は提供承諾書を用いて、事前に寄託者（筑波大学・三輪佳宏 <ymiwa@md.tsukuba.ac.jp>）の承諾を得る。
iRFP Tg #867
iRFP Tg #867
<a href='https://brc.riken.jp/mus/pcr10068'>Genotyping protocol -PCR-</a>
RBRC10068